Cross Immunity among the 49.8 KDa Pili Subunit Hemagglutinin Proteins and 7.9 KDa Pili Subunit Anti Hemagglutinin Proteins of Shigella spp

In a recent report, 49.8 kDa pili subunits haemagglutination
(HA) protein of S. dysenteriae which proved to be an adhesive
molecule and allow it to be developed as a vaccine. The
purpose of this study was to obtain more information in the
immunological response to the 49.8 kDa pili subunit protein
Shigella spp. Pili subunit proteins Shigella spp. isolated
according to our procedure manual. The molecular weight
(MW) of 49.8HA and anti-HA 7.9kDa were identified using
SDS-PAGE. The immunological response was detected using
western blotting and dot blotting. Statistical analysis using
ANOVA. The results of tests using Western blotting and dot
blot method have been found the same immunological reaction
between polyclonal antibodies pili 49.8kDa subunits protein of
S. dysenteriae against 49.8 pili protein subunits of S.
dysenteriae and 49.8 kDa pili protein subunits of S. boydii and
polyclonal antibodies pili7.9kDa subunit protein of S.
dysenteriae against 7.9 kDa pili protein subunits of S.
dysenteriae and 7.9 kDa pili protein subunits of S.boydii 7.9
kDa as well, but not identical immunological reaction between
7.9 kDa pili protein subunits S. flexneri, S. sonnei and 49.8kDa
pili protein subunits of S. flexneri, S. sonnei. There are
similarities epitopes between the 7.9 kDa protein pili subunits
of S. dysenteriae with 7.9 kDa protein pili subunits of S. boydii
and the 49.8 kDa protein pili subunits of S. dysenteriae with
49.8 protein pili subunits of S. boydii as well, but differences
epitopes with the 7.9kDa and 49.8 kDa protein pili subunits of
S. flexneri and S. sonnei